New Methods in Cardiovascular Biology A Novel Mitochondrial KATP Channel Assay
نویسندگان
چکیده
mKATP activity, and used this assay probe several aspects of mKATP function. The following key observations were made. (1) Time-dependent run down of mKATP activity was reversed by phosphatidylinositol-4,5bisphosphate (PIP2). (2) Dose responses of mKATP to nucleotides revealed a UDP EC50 of 20 mol/L and an ATP IC50 of 5 mol/L. (3) The antidepressant fluoxetine (Prozac) inhibited mKATP (IC50 2.4 mol/L). Fluoxetine also blocked cardioprotection triggered by IPC, but did not block protection triggered by a
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